FREETOWN, Sierra Leone (AP) — The deadly Ebola virus has infected two people in what was the last untouched district in Sierra Leone, the government said Thursday, a setback in efforts to stop the spread of the disease in one of the hardest-hit countries.

The Emergency Operations Center in its report covering Wednesday announced the two Ebola cases in the Koinadugu district, in Sierra Leone’s far north, which had taken aggressive measures to keep the virus out of its mountainous territory since the outbreak early this year.