President Joe Biden sat down for his second prime-time interview since his disastrous debate last month, but over the course of the roughly 18-minute interview with NBC News’s Lester Holt, the president appeared to lose his cool.

As the interview continued, Biden became more testy as he was asked about the assassination attempt on his opponent, former President Donald Trump, along with questions from Democrats about whether the president should continue to run after his shaky debate performance. These are six of the moments when Biden appeared to lose his patience with Holt during the interview.